The D-DEAR Framework

A five-stage workflow for reproducible quantitative science.
MIQE showed us what to document. D-DEAR shows us how to work.

Simple SVG + CSS Animation

DefineRDES
DesignMIQE
ExecuteMIQE
AnalyzeMIQE
ReportMIQE+FAIR

D3.js with Particles, Curved Paths & Interactive Hover

Integration Flow

Pipeline Flow (with branching)

Define

Research question & intent

Research question
Goals
References
Design

Plate layout & templates

Samples
Assays
Plate layout
Execute

Wet-lab protocol & data import

Sample prep
PCR run
Data import
Analyze

QC, normalization & stats

QC checks
Normalization
Statistics
Report

Traceable, publication-ready reports

Audit trail
Figures
Final report

The D-DEAR Manifesto

We've wasted too many scientific hours reconstructing what should have been recorded.

We've rerun too many experiments because the metadata lived in someone's head.

We've published too many methods sections that couldn't actually reproduce the method.

Reproducibility shouldn't be this hard.

DefineA vague question produces vague results. Clarity before pipettes.
DesignReproducibility is not achieved in analysis. It's built into the plate.
ExecuteProtocol without documentation is ritual, not science.
AnalyzeIf you can't explain the calculation, you can't defend the conclusion.
ReportA result that can't be retraced isn't evidence. It's anecdote.

Every stage traceable.

Every result defensible.

Every experiment worth building on.

Building on Established Standards

D-DEAR doesn't replace existing standards. It operationalizes them into a unified workflow.

MIQE

Minimum Information for Publication of Quantitative Real-Time PCR Experiments. The gold standard for qPCR reporting.

FAIR

Findable, Accessible, Interoperable, Reusable. Principles for scientific data management and stewardship.

RDES

Recommended Data and Experimental Standards. Guidelines for experimental design documentation.

Ready to implement D-DEAR?

Clarida is the platform that makes D-DEAR effortless.