Blog
What we’ve learned building qPCR software for 15 years. And what we’re building next.
Practical insights on qPCR workflows, data quality, and lab software - from the creators of qbase+ and the MIQE guidelines.
Latest posts
Is it better to pipet duplicates or triplicates in real-time PCR?
Reproducible Cq values give a false sense of security if upstream steps are uncontrolled. Understanding all sources of variation is key before deciding on duplicates or triplicates.
From sample list to plate layout in one click
You have 12 samples, 3 assays, and triplicates. Do you sketch the layout on paper or copy last week's Excel? Clarida generates it for you. One click for a sensible default, four settings when you need control.
How to bring light to PCR: TaqMan probe or SYBR Green dye?
Dye-based assays are perfect for many qPCR applications. When you understand the true advantages of probe-based assays, you can reduce cost while keeping quality high.
Why is the PCR amplification efficiency still ignored?
Most qPCR results assume 100% amplification efficiency. When that assumption is wrong, the published fold differences are wrong too. Here is why the actual efficiency cannot be ignored.
Mini qPCR playbook: 11 proven tips on normalization & biostatistics
Improper normalization and weak statistics are among the top causes of irreproducible qPCR results. 11 actionable tips distilled from over two decades of best practice.
Clarida early access is live
We wrote about the PCR workflow problem. Today, we are inviting you in. Early access is free - try it, break it, tell us.
Functional qPCR instrument validation
Even well-calibrated qPCR cyclers can introduce unnoticed measurement errors. A practical, low-cost run homogeneity test can catch them before your data suffers.
The Clarida manifesto: fixing the PCR workflow
The science is mature, but the workflow is broken. We believe PCR researchers deserve better than juggling Excel, cycler software, and fragmented analysis tools.